Sample (downsample) repertoires using different approches.
repSample(
.data,
.method = c("downsample", "resample", "sample"),
.n = NA,
.prob = TRUE
)The data to be processed. Can be data.frame, data.table, or a list of these objects.
Every object must have columns in the immunarch compatible format. immunarch_data_format
Competent users may provide advanced data representations: DBI database connections, Apache Spark DataFrame from copy_to or a list of these objects. They are supported with the same limitations as basic objects.
Note: each connection must represent a separate repertoire.
Character. Name of a sampling method. See "Details" for more details. Default value is "downsample" that downsamples the repertoires to the number of clones (i.e., reads / UMIs) that the smallest repertoire has, if user doesn't set any value to the ".n" argument.
Integer. Number of clones / clonotypes / reads / UMIs to choose, depending on the method. Set NA to sample repertoires to the size of the smallest repertoire in the ".data".
Logical. If TRUE then samples the clonotypes with probability weights equal to their number of clones. Used only if ".method" is "sample".
Subsampled immune repertoire or a list of subsampled immune repertoires.
If .method is "downsample" then repSample chooses .n clones (not clonotypes!) from the input repertoires without any probabilistic simulation,
but exactly computing each choosed clones. Such approach is is more consistent and biologically pleasant than
an output from the function if .method is "resample".
If .method is "resample" then repSample uses multinomial distribution to compute the number of occurences for each cloneset.
then it removes zero-number clonotypes and return the resulting data frame. Probabilities for rmultinom for each cloneset
is a percentage of this cloneset in the "Proportion" column. It's a some sort of simulation of how clonotypes are chosen from the organisms.
if .method is "sample" then repSample chooses .n clonotypes (not clones!) randomly. Depending on the
.prob argument, the function chooses clonotypes either according to their size (if .prob is TRUE, by default),
or each clonotype has an equal chance to be choosed (if .prob is FALSE). Note that sampling is done without replacing.
data(immdata)
# Downsampling to 1000 clones (not clonotypes!)
tmp <- repSample(immdata$data[[1]], .n = 1000)
sum(tmp$Clones)
#> [1] 1000
# Downsampling to 1000 clonotypes
tmp <- repSample(immdata$data[[1]], "sample", .n = 1000)
nrow(tmp)
#> [1] 1000
# Downsampling to the smallest repertoire by clones (not clonotypes!)
tmp <- repSample(immdata$data[c(1, 2)])
sum(tmp[[1]]$Clones)
#> [1] 8500
sum(tmp[[2]]$Clones)
#> [1] 8500
# Downsampling to the smallest repertoire by clonotypes
tmp <- repSample(immdata$data[c(1, 2)], "sample")
nrow(tmp[[1]]$Clones)
#> NULL
nrow(tmp[[2]]$Clones)
#> NULL